Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Preparing Plates and Performing the Pure Dye Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 73
Notes
6
Preparing Plates and Performing the Pure Dye Calibration
Selecting the Dye
1. In the Pure Spectra Calibration Manager:
a. In the Dye List field, select a pure dye to
calibrate.
b. Click .
c. If you are prompted to disconnect the plate
document, click .
d. A message prompts you to load the plate.
Do not click Yes or No at this time.
Preparing and Loading a Pure Dye Plate
1. Remove the appropriate pure dye plate from its
packaging.
IMPORTANT! Do not discard the packaging for
the pure dye plate. The pure dye plate can be
used up to three times if it is stored in its original
packaging sleeve.
2. Standard plates only: Vortex the plate for
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
ba
Click

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)