Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Preparing Plates and Performing the Pure Dye Calibration
74 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
3. Briefly centrifuge the pure dye plate in a
centrifuge with a plate adapter (<1500xg).
4. Verify that the dye standard in each well of the
pure dye plate is at the bottom of the well.
If not, centrifuge the plate again at a higher speed
and for a longer period.
5. Verify that the pure dye plate that you are about
to load matches the dye selected in the Pure
Spectra Calibration Manager
.
6. Load the plate in the instrument as described in
“Loading the Plate” on page 40.
Note: When closing
the instrument tray,
apply pressure to the
right side of the tray
and at an angle.
Performing the Pure Dye Calibration
In the dialog box that prompts you to load the plate
(see step 1d on page 73, click , then wait for
the run to complete (~5 minutes).
00:00:05<1500× g
Correct Incorrect
Liquid is at
bottom of well.
• Not centrifuged with enough force,
or
• Not centrifuged for enough time
Dye label
Notched
corner
Well A1
12
GR2475
GR2475
7500
keyed corner
A1
A
B
C
D
E
F
G
H
123456789101112
A
1
7300/7500 System
Standard plate
7500 Fast System
Fast plate
Press
here
>
00:05:00
Click

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)