Applied Biosystems 7500 User Manual

Appendix A
Recommended Maintenance Schedule
106 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Semi-Annual
Maintenance
Tasks
• Perform a background calibration (see page 49)
• Perform an optical calibration on 7500/7500 Fast systems (see page 59)
• Perform a region of interest (ROI) calibration (see page 35)
• Perform a pure dye calibration (see page 67)
Note: You can run a background calibration to check for contamination.If any parts of
the optics are replaced or moved, you must run a background calibration, an ROI
calibration, an optical calibration (7500/7500 Fast only), and a pure dye calibration.
Miscellaneous
Maintenance
Tasks
Perform the following tasks as needed to resolve problems as they arise:
• Decontaminate the sample block (see page 108)
• Move the 7300/7500/7500 Fast system (see page 115)
• Replace the halogen bulb (see page 117)
• Replace the instrument fuses (see page 122)
• Update the Microsoft Windows operating system (see page 124)
6 Months
January
Su M T W Th F S Su M T W Th F S
July

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)