Applied Biosystems 7500 User Manual

Chapter 4 Performing the Regions of Interest (ROI) Calibration
Overview
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 37
Notes
4
Purpose of the
ROI Calibration
A regions of interest (ROI) calibration maps the positions of the wells on the sample
block so that the software can associate increases in fluorescence during a run with
specific wells of the plate. Because the instrument uses a set of optic filters to distinguish
the fluorescence emissions gathered during runs, you must generate a calibration image
for each individual filter to account for minor differences in the optical path.
ROI calibration image

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)