Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Overview
70 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Custom Dyes The 7300/7500/7500 Fast instrument supports the detection of custom pure dyes (dyes
other than those provided by Applied Biosystems).
Custom dyes must fluoresce within the spectral range measured by the instrument:
• 500 to 650 nm for 7300 systems
• 500 to 700 nm for the 7500/7500 Fast systems
To add custom pure dyes to the Pure Dye set for your instrument, see “Creating a
Custom Pure Dye Plate” on page 127.

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)