Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Evaluating Pure Dye Calibration Spectra
82 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
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Table 2 Spectra for pure dyes supported by the 7500 system
Instrument Pure Dye Filter Peak (nm)
7500 System FAM A ~520 nm
JOE B ~550 nm
NED C ~550 nm
ROX D ~610 nm
SYBR
Green
A ~520 nm

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)