Applied Biosystems 7500 User Manual

Appendix A
Decontaminating the Sample Block
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 109
Notes
A
Cleaning the Sample Wells
IMPORTANT! Wear powder-free
gloves when you perform this
procedure.
1. Identify the contaminated wells of the sample
block (see “Troubleshooting” on page 64).
2. Remove the plate and the tray holder.
3. Close the tray. Apply pressure to the right side of
the tray and at an angle.
4. Manually raise the block from the ROI Inspector
window:
a. Click (or select FileNew).
b. In the New Document wizard,
click .
c. In the SDS software, select
InstrumentCalibrate.
d. In the warning dialog box, click to
lower the sample block.
The ROI Inspector dialog box opens.
e. In the ROI Inspector dialog box,
click .
5. Power off, then unplug the 7300/7500/7500 Fast
system. Allow it to cool for 15 min.
Press
here
>00:15:00

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)