Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
95
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
Prepare for the
Calibration
PHYSICAL INJURY HAZARD. During instrument operation, the
temperature of the sample block(s) can exceed 100 ° C. If the instrument has been used
recently, keep your hands away until the sample block(s) reaches room temperature.
IMPORTANT! Wear powder-free gloves when you handle the background plate.
1. If you have not already done so, use the StepOne system cable to connect the:
• Yellow Ethernet port ( ) of the instrument to the
• Ethernet port ( ) of the computer running the StepOne
™
software
IMPORTANT! Do not connect the StepOne system cable to the blue LAN Port ( ).
2. Get the background plate from the spectral calibration kit in the freezer.
3. Allow the
background plate to warm to room temperature (at least 5 min).
4. Remove the background plate from its packaging.
IMPORTANT! Do not discard the packaging sleeve. The background plate can be
used up to three times if it is stored in its original packaging sleeve.
5. Vortex the background plate for 5 sec.
6. Centrifuge the background plate for 2 min at less than1500 rpm.
IMPORTANT! The background plate must be well mixed and centrifuged.
Ethernet

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English