Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Get_Started.fm
Chapter 1 Get Started
About the StepOne
™
and StepOnePlus
™
Systems
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
4
Notes
Filters and
Supported Dyes
StepOnePlus
™
System Filters and Dyes
The StepOnePlus
™
instrument features a four-color filter set that supports the following
Applied Biosystems dyes: FAM
™
dye, JOE
™
dye, NED
®
dye, ROX
™
dye, TAMRA
®
dye,
VIC
®
dye, and SYBR
®
Green dye. The following figure shows the emission spectrum for
each dye, and the filter at which each dye is read.
StepOne
™
System Filters and Dyes
The StepOne
™
instrument features a three-color filter set that supports the following
Applied Biosystems dyes: FAM
™
dye, JOE
™
dye, ROX
™
dye, VIC
®
dye, and SYBR
®
Green dye. The following figure shows the emission spectrum for each dye, and the filter
at which each dye is read.
About the
VeriFlex
™
Technology
The StepOnePlus instrument contains six independently thermally regulated VeriFlex
™
blocks to help you optimize your thermal cycling conditions. You can set a different
temperature for one or more of the VeriFlex blocks, creating up to six different zones for
samples, or you can set the same temperature for each of the VeriFlex blocks.
31Filters 4
Wavelength
(nm)
Emission
Spectra
Dyes
2
~520 nm
• FAM
• SYBR Green
• JOE
• VIC
• ROX
~610 nm
• NED
• TAMRA
~580 nm~550 nm
500 600 700
Filters 1 2 3
Wavelength
(nm)
Emission
Spectra
Dyes
~520 nm
• FAM
• SYBR Green
• JOE
• VIC
• ROX
~610 nm~550 nm
500 600 700

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English