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Applied Biosystems StepOne User Manual

Applied Biosystems StepOne
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DRAFT
September 25, 2007 1:07 am, 4376782_TOC.fm
v
Applied Biosystems StepOne
and StepOnePlus
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Chapter 6 Maintain the System . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Regular Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Perform a Spatial Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Perform a Background Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Perform a Dye Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Archive and Back Up Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Infrequent Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Decontaminate the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Move the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Replace the External Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Ship the Instrument for Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Update the Windows Operating System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Update the StepOne
Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Appendix A How to Troubleshoot the Installation . . . . . . . . . . . . . . . 127
StepOne
Software Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
Network Connection Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
Background Calibration Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
Dye Calibration Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153

Table of Contents

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Applied Biosystems StepOne Specifications

General IconGeneral
BrandApplied Biosystems
ModelStepOne
CategoryLaboratory Equipment
LanguageEnglish

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