DRAFT
September 25, 2007 1:07 am, 4376782_IX.fm
Index
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
158
set up
computer 28
, 51
instrument 20
site requirements
computer 28
, 52
instrument 15
slope 147
SNP 147
SNP assay 147
SNP Assay Library 147
software
administrator rights requirement 34
, 57
antivirus 8
, 47, 71
encryption 8
, 47, 71
file compression 8
, 47, 71
firewall 8
, 47, 71
installation 31
–34, 54–57
installation (third-party) 8
installation, administrator rights requirement 34
, 57
optional 7
prepare for use 58
prepare operating system 31
–33, 54–56
shipped with the system 19
system optimization 8
software, third-party 8
spatial calibration 90
–93, 147
analyzing the data 93
perform 92
prepare 90
–91
purpose 90
specification, installation 39
, 44, 61, 69
spectrum, system dyes 108
, 109
stage 147
standalone layout 147
connect to a network 74
description 10
example network layout 75
installation 50
–71
installation workflow 50
when to install 50
standard
curve 147
curve method 148
standard dilution series 148
standard quantity 148
standards
EMC xx
safety xx
standard 147
starting quantity 148
statistics, instrument 89
step 148
storage devices, backup 7
surge protector, requirements 7
, 20, 27, 51
SYBR
®
Green I dsDNA Binding Dye 4, 101
reagents 148
spectral profile 108
, 109
symbols, safety xii
system
chemistry kit 18
components 17
data collection 3
documentation 19
packing kit 17
–18
software 19
system dyes 101
, 108, 109, 149
T
TAMRA
®
dye 4, 101
spectral profile 109
TaqMan
®
Fast Universal PCR Master Mix, No
AmpErase
®
UNG 38, 60
TaqMan
®
RNase P Instrument Verification Plate 18, 38,
60
See also RNase P experiment.
TaqMan
™
reagents 149
target 149
color 149
library 149
task 149
Technical Support, contacting x
temperature
plot 150
requirement, computer 28
, 52
requirement, instrument 15
verification service 89
template 150
test
optics verification 89
run cycle performance 89
third-party software for incompatibilities 8
text conventions viii
thermal profile 150
third-party software 8
threshold 150
threshold cycle C
T
150
Tm. See melting temperature.
Tools menu, maintenance tests 89
touchscreen 150
training, information on x
transfer data 66
download experiment from instrument 84
send experiment to instrument 84
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