DRAFT
September 25, 2007 1:07 am, 4376782_IX.fm
Index
157
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
point 143
positive control 143
post-PCR read 143
power line regulator 7
, 27, 51
power options settings, for the software 31
, 54
power requirement
computer 28
, 52
instrument 15
preparation, system installation 5
prepare
background calibration 95
–96
dye calibration 103
–104
RNase P experiment 40
, 62
spatial calibration 90
–91
pre-PCR read 143
primer mix 143
primer/probe mix 144
protective devices, electrical 7
pure dye calibration. See dye calibration.
pure dyes 144
Q
quantitation method 144
quantity 144
quencher 144
QuickStart 144
R
R2 value 45, 70, 144
radioactive waste, handling xvii
ramp 144
ramp speed 144
raw data plot 144
reaction mix 145
reagents 145
real-time PCR 145
reference sample 145
refSNP ID 145
regression
coefficients 145
line 145
regulator, power line 7
, 27, 51
reject well 145
relative standard curve method 145
remote monitoring 74
, 145
enable notification settings 85
guidelines 82
monitor instrument 82
set up (computer) 80
removing outliers from RNase P runs 43
, 68
repetitive motion, safety xix
replicate group 146
replicates 146
reporter 146
requirements
computer 6
, 28, 52
instrument 15
See also materials required.
reverse primer 146
reverse transcriptase 146
Rn. See normalized reporter.
RNase P experiment 38
, 60
analyze 43
–45, 68–70
materials required 38
, 60
perform 41
, 63
prepare 40
, 62
purpose 38
, 60
R2 value 45
, 70, 144
transfer data 66
when to perform 38
, 60
ROX
™
system dye 4, 101, 146
spectral profile 108
, 109
rs number 146
run cycle performance test 89
run method 146
S
safety
before operating the instrument xiv
biological hazards xix
chemical xv
chemical waste xvii
conventions xi
electrical xviii
ergonomic xix
guidelines xv
, xvii
instrument operation xiv
labels, on instruments xiii
moving and lifting instrument xiv
moving/lifting xiv
repetitive motion xix
standards xx
symbols, on instruments xii
workstation xix
sample 146
sample block, decontaminate 115
–117
Sample DNA (105) 146
Sample Library 146
Sample or Standard 146
sample/SNP assay reaction 146
sample/target reaction 146
screen resolution, for the software 32
, 55
screen saver, for the software 31
, 54
security software 8
, 47, 71
serial factor 147
series 147
service pack, updates 124
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