Applied Biosystems StepOnePlus User Manual

DRAFT September 25, 2007 1:07 am, 4376782_IX.fm
Index
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
156
M
MAC address, instrument 78
made-to-order assays 142
maintenance
archiving files 113
back up, experiments 113
decontamination 115
–117
disk space, checking 113
infrequent procedures 114
notifications 88
optics verification 89
required connection 88
run cycle performance test 89
schedule 88
statistics, instrument 89
temperature verification service 89
using this guide 5
view information 89
managed data network service. See mDNS.
manual baseline 142
manual C
T
142
materials required, to
analyze the RNase P experiment 65
create a background plate 99
decontaminate the sample block 115
install the system 6
move the instrument 118
network the instrument 78
perform a background calibration 94
perform a custom dye calibration 110
perform a dye calibration 100
perform a spatial calibration 90
perform an RNase P experiment 38
, 60
replace the instrument fuses 120
set up remote monitoring 80
set up the computer 27
, 51
ship the instrument 122
materials, shipped with the system 17
mDNS/DNS compatibility 76
Media Access Code address. See MAC address.
melt curve stage 142
melt curve 142
melting temperature (Tm) 142
menu commands, conventions for describing viii
monitor the instrument. See remote monitoring.
moving and lifting, safety xiv
moving guidelines 16
, 28, 52
MSDSs
description xv
obtaining x
, xvi
multicomponent plot 142
N
NED
®
system dye 4, 101
spectral profile 109
negative control (NC) 142
negative control-blocked IPC wells 142
negative control-IPC wells 142
network 74
–86
example layouts 75
guidelines 77
set up workflow 74
setup 74
using this guide 5
no amplification control (NAC) 142
no template control (NTC) 142
nonfluorescent quencher-minor groove binder (NFQ-
MGB) 143
normalized quantity 143
normalized reporter (Rn) 143
notification settings, enable 85
O
omit well 43, 68, 143
online Help. See Help system
operating system
prepare 31
–33, 54–56
update 124
optics verification 89
optimization software 8
outlier 143
outliers 43
, 68
overvoltage category (rating) xviii
P
packing kit, contents 17–18
packing material, removal 21
passive reference 143
password, default for computer 30
, 53
perform
background calibration 96
dye calibration 105
–106
RNase P experiment 41
, 63
spatial calibration 92
placement
computer 28
, 52
instrument 16
plate layout 143
plates
background plate 94
background plate, custom 99
MicroAmp plates 17
, 18
number of reuses 95
RNase P plate 18
, 38, 60
spectral calibration plate 90
, 100

Applied Biosystems StepOnePlus Specifications

General

Brand	Applied Biosystems
Model	StepOnePlus
Category	Laboratory Equipment
Language	English