184 | Appendix E: Instructions for Programming a New Assay
BioTek Instruments, Inc.
STEP COMMENTS
SAMPLES: 91 User-defined; recommendation is to fill
the entire plate
SAMPLE REPLICATES: 01
Use the MAP and MATH keys to
create the control validation formula:
FORMULA
VAL
CONTROL:
PC;x > 1.200
NO. OF REPLICATES: 01
NC;x < 0.300
NO. OF REPLICATES: 01
When defining control validation
formulas, use “PC” to indicate the
criterion for each of the PC replicates,
and “PC;x” to indicate the average of the
Positive Control replicates.
To create the plate transformation for CALC,
divide the ANA result by a cutoff standard.
FORMULA
*MORE
TRANS-VAR
SCOPE VARIABLE (SMP or OD) Select OD to advance to the formula
definition screen and define the
transformation variable (Tvar)
FORMULA
CTL1;X Defining TRANS VAR = CTL1;X isolates
the OD value for CTL1;X for use in
transformation
TRANS:
FORMULA: (OD/TVAR)*10 Converts all OD values on plate to
”ANA Result” per kit insert instructions
To define a cutoff formula for Positive and
Negative calls:
FORMULA
CUTOFF: 10.0
GREYZONE: 00%
SAMPLE > CUTOFF + 00%: POS
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