Define (Method, Map, Formula and Curve) | 57
ELx800 Operator’s Manual
Blank Map Definitions
AIR performs an initial reading on “air” just prior to the plate read,
and uses that value as the blank value. This value is subtracted from
each well on the plate.
FULL enables a single blank well or an average of blank wells to be
subtracted from the whole plate.
CONST (Constant) allows entry of a user-specified absorbance value.
This value will be subtracted from each well on the plate.
Tip for using CONST: Use a blank value from the first plate, or a
blanking plate, to save space on subsequent assay plates.
ROW enables a single blank well or an average of blank wells to be
selected for each row. The maximum number of blanks is 48. The
blank (or average) will be subtracted from each well in the row. Use
manual mapping to position blanks, controls, standards, and
samples.
COLUMN enables a single blank well or an average of blank wells to
be placed in each column. Since the maximum number of blanks is
12, and if all 12 columns are used, each column can have only one
blank. Manual mapping is recommended in this case. Replicates
follow in the same column as the first well of each sample, even if
the ACROSS direction has been specified for replicates.
P-ACROSS enables a blank in every even-numbered column to be
subtracted from the well to the left of it in every odd column.
Manual mapping is recommended to set up the appropriate map by
placing the standards, controls, and samples in only the odd
columns.
P-DOWN enables a blank in the B, D, F and H rows to be subtracted
from the well above in the A, C, E and G rows. Manual mapping is
recommended to set up the appropriate map by placing the
standards, controls, and samples in only the A, C, E, and G rows.
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