72 | Chapter 3: Operation
BioTek Instruments, Inc.
Number of Required Controls/Blanks
If a control or blank validation formula is defined, enter the number of
valid controls or blanks that must meet the criteria established by that
formula.
P C : N U M B E R O F V A L I D
R E P L I C A TES REQUIRED: 0 2
Use the numeric keys to enter the NUMBER OF REQUIRED
CONTROLS. The range is 1 through the number of defined replicates
of a control or blank.
Press the CLEAR key to clear the displayed value.
Press ENTER to save the displayed value and advance to the next
screen, or use the
Previous Screen key to move backward through
the menu structure.
Assay Validation Formulas
Assay Validation formula(s) establish a set of criteria used to determine
whether or not an assay can be considered valid. If the criteria are not met,
results are considered suspect, and the message “RESULTS INVALID!
Assay validation failed” appears on results reports.
Up to 4 assay validation formulas can be defined.
Define the plate map (via DEFINE MAP) before creating assay
validation formulas.
Examples
If an assay protocol states that for the assay to be valid:
The mean of the negative controls must be less than 0.100. The
formula is: NC;x<0.100
The mean of the positive controls must be greater than the mean of
the negative controls. The formula is: PC;x>NC;x
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