Calibrating the Tubing Cassette Gravimetrically | 103
BioTek Instruments, Inc.
9 Measure and record the Gross mass (the mass of the strip and fluid) of each
strip.
The worksheet subtracts the
Tare mass from the Gross mass to obtain the
Actual mass dispensed into each strip. It subtracts the Expected mass from
the
Actual mass. From these values, it displays a “Correction”
recommendation for the number of turns of the screw and the current
Accuracy percentage.
10 Depending on the result of (Actual mass – Expected mass), use the screwdriver
shipped with the MicroFlo to adjust the tube’s tension screw:
Correction (turns): the Gravimetric Cassette Calibration Worksheet
displays a recommended number of turns. Turn clockwise for a positive
number; turn counter-clockwise for a negative number.
(Actual – Expected) = Positive Value (Actual – Expected) = Negative Value
When the difference between the
expected and the actual mass is positive
(too much fluid dispensed) turn the
adjustment screw clockwise to increase
tension on the tubing.
When the difference between expected and
actual mass is negative (not enough fluid
dispensed) turn the screw counter
clockwise to decrease tension.
Figure 4: Use the screwdriver shipped with the MicroFlo to adjust the tension in the tubing.
11 Repeat the process, beginning at Step 5, until the desired Accuracy % is
achieved.
To Next Page
Loading...