- 31 -
Optimizing the Field of View and Focus
Start the VisualSpreadsheet software by double-clicking on the icon located on the desktop. When
prompted, select the appropriate magnification. A notification will open to remind the operator to
ensure that the correct objective and collimator/diffuser (if required) is installed.
Figure 16. VisualSpreadsheet Icons
To assist the user, VisualSpreadsheet can be configured to display a Status Bar at the bottom of the Main
Window (see Figure 17). To enable this feature navigate to Preferences > Main Window Settings >
Layout > Status Bar > Show Status Bar. The Status Bar is color coded to match the color line on the
objective. The colors are black (1/2X), Blue (1X), grey (2X), red (4X), yellow (10X), and green (20X).
Figure 17. Example of the Status Bar displayed in the Main Window
Open Setup and Focus window
Entering VisualSpreadsheet’s Setup and Focus Mode (Setup > Setup and Focus [F6]) enables the user to
view live camera images (of the flow cell). This can be used to assist with the following tasks:
· Positioning the flow cell vertically and horizontally into the camera’s field of view
· Inspect the flow cell for imperfections
· Determine the effectivness of flow cell cleaning procedures
· Focusing on particles
· Live viewing of the sample of interest currently in the flow cell
To Next Page
Loading...
