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Course/Rough focus
To coarse focus the field of view, loosen the rail lock thumb screw (see Figure 18). Slide the entire
Flow Cell Holder Assembly to bring any part of the flow cell into focus. When the flow cell has been
located, tighten the rail lock thumb screw.
WARNING: Extra care should be used with the 20X objective, to prevent it from coming in contact with
(and potentially breaking) the 50 µm flow cell.
Position adjustment of the flow cell
Fine adjustments and positioning of the flow cell is possible by use of the X- and Y-axis positioning
screws (Figures 18 & 19). This adjustment should be done, for example, to move a scratch in the glass
wall of the Flow Cell to a position outside of the field of view. For fine vertical movement of the Flow
Cell, turn the Y-positioning screw. For fine horizontal movement of the Flow Cell, turn the X-
positioning thumb screw.
Figure 18. Course and Fine focus adjustment screw and knobs (also refer to Figure 2, letter M)
Course Focus Adjustment Screw
X-Axis Adjustment Knob
Y-Axis Adjustment Knob