- 33 -
Figure 19. Course and Fine focus adjustment screw and knobs for the open bench top model
Adjust the field of view illumination (if necessary)
There are two ways to adjust the light intensity passing through the flow cell to the objective (note that
the flow cell should contain sample diluent or sample of interest before adjusting illumination and
focus). The first method utilizes the software control of the camera. The camera settings that control
the light intensity can be accessed from two different menus in VisualSpreadsheet. The first menu is
located via Setup > Context > Camera (tab) and Timing (tab). The second menu is accessed by selecting
Camera from the Setup menu in the Setup and Focus (F6) window (See Figure 20). The advantage of
accessing the menu with the second method is that the Setup and Focus window will remain open and
visible. Any adjustments that are entered and applied will be displayed to the user, allowing for instant
feedback.
Figure 20. Setup and Focus Mode field of view window
X-Axis Adjustment Knob
Y-Axis Adjustment Knob
Course Focus Adjustment Screw
Micrometer (Fine Focus)