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LI-COR C-DiGit User Manual

LI-COR C-DiGit
121 pages
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Blot Scanner
User Guide
®

Table of Contents

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LI-COR C-DiGit Specifications

General IconGeneral
ModelC-DiGit
Dynamic range>4 orders of magnitude
Scan area25 x 25 cm
Dimensions43 x 56 x 18 cm (17 x 22 x 7 inches)
Weight11.4 kg (25 lb)
ApplicationWestern blot documentation
Detection MethodFluorescence
Resolution21 μm
Power Requirements100–240 VAC, 50/60 Hz
Image FormatTIFF
ConnectivityUSB
Laser Wavelengths680 nm and 780 nm

Summary

Notes on Safety

Equipment and Manual Markings

Explains safety symbols and warning levels used in the manual.

Regulatory Statements

Provides Federal Communications Commission (FCC) and CE marking compliance information.

Introduction

About the C-DiGit Blot Scanner

Overview of the C-DiGit Blot Scanner's purpose and functionality.

What’s Included and Lab Placement

Lists included items and provides guidelines for scanner placement in the lab.

Safety and Operational Guidelines

Covers space, electrical, and chemical safety considerations for operation.

Instrument Panel Controls

Details the front and back panel components and indicators of the instrument.

Scanning Surface and Sample Handling

Information on the scanning surface, safety, and correct sample placement.

Quick Start Tutorial

Initial Setup and Software Installation

Steps for unpacking, placing the scanner, and installing Image Studio software.

System Connection and Driver Setup

Guides on connecting USB/power cables and installing necessary drivers.

Software Configuration and Verification

Setting up the work area, starting Image Studio, and connecting the scanner.

Target Image Acquisition and Adjustment

Procedure for acquiring and adjusting the C-DiGit target image for verification.

Scanning Membranes

Membrane Handling and Preparation

General tips for handling and preparing membranes for scanning.

Blocking and Antibody Procedures

Guidance on using blocking buffers and primary/secondary antibodies.

Washing and Substrate Application

Instructions for washing membranes and applying detection substrates.

Imaging Process and Optimization

Details on the imaging process, including adjustments for optimal results.

Image Studio Software Operations

Software Interface Overview

Description of the main window layout, including ribbons and tables.

File Management and Data Export

Operations for saving, exporting, importing images, and managing files.

Image Viewing and Manipulation

Techniques for viewing, adjusting, and manipulating image data.

Data Analysis Tools

Using shapes, annotations, and quantification for data analysis.

Table Management and Export

Managing images and shapes tables, including sorting and filtering.

Computer Connections and Networking

Connection Methods

Explains how to connect the scanner via USB, Ethernet, or LAN.

Maintenance and Troubleshooting

Routine Maintenance Guidelines

Provides recommendations for cleaning and inspecting the instrument.

Hardware Troubleshooting

Addresses issues related to hardware, connectivity, and errors.

Chemistry and Software Troubleshooting

Solutions for background, signal, and Image Studio software problems.

Specifications

Windows XP Installation Instructions

Software and Hardware Setup

Steps for installing software, connecting cables, and powering on the scanner.

Driver Installation and Network Configuration

Guides through the new hardware wizard and network connection setup.

Connection Troubleshooting for Windows XP

Provides steps to diagnose and resolve connection issues in Windows XP.

Training Video Links