Milestone Srl MA213-003 – DMA-80 EVO – User Manual
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4. PRACTICAL EXAMPLE OF A CALIBRATION AT LOW RANGE (0.1 ng)
This example shows how to properly calibrate the DMA-80 evo at low range, similar approach could be
used to calibrate the system at higher levels, see dedicated chapter in this manual.
The example of calibration has been designed to quantify 0.1 ng, for a working range from 0,1 ng to 1,5
ng.
- Prepare the blank and the standards as explained above.
- System must be clean
- The calibration to quantify 0,1 ng requires a calibration point lower than 0,1 ng, for example 0.05 ng.
- Never force to 0 the calibration (BV correction: OFF) in cell 0, but use a real blank as described in the
calibration paragraph. In Cell 1 and 2 calibration can be forced to zero.
- To maximize the performance of the system and its linearity at low range, the max ABS value has to be
set to a slightly higher absorbance of the 1.5 ng point (last point of this calibration in cell 0). In this specific
example, the “Absorbance limit of cell 0” was set to 0.1500, as the highest calibration point in cell 0 (1.5
ng) was 0.1400 ABS.
Below is reported the obtained calibration: