Odyssey LI-COR - Entering Scan Parameters

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Entering Scan Parameters
Scan parameters, such as resolution and scan area, can all be entered
individually or loaded from stored sets called
Presets . For most scans
it is easiest to load Preset parameters and then edit individual para-
meters, like scan area, to suit the current scan.
Loading Preset Parameters
3) Choose a set of scan parameters from the Preset drop-down list.
If you are not using Preset parameters skip to Step 4.
The Odyssey
®
software initially has three sets of Preset parameters –
one for membranes and two for gels.
The default Presets generally produce good results, but you will
probably want to save Presets that more closely match your needs.
Creating Presets is discussed later in this chapter.
LI-COR Presets Membrane DNA Gel Protein Gel Microplate
Resolution 169 169 169 169
Quality medium medium medium medium
Intensity 5.0 8.0 5.0 5.0
Channels 700, 800 700, 800 700, 800 700, 800
Scan Origin 0,0 0,0 0,0 0,0
Scan Size 10,10 10,10 10,10 13,9
Focus Offset 0.0 2.0 0.5 3.0