Pickering Laboratories Pinnacle PCX - Page 103

6.2-13
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
METHOD 8: 1154110T COLLAGEN HYDROLYSATE SODIUM (4.6X110 MM)
GUARD COLUMN: GARD COLUMN TEMP: SEE GRADIENT HPLC FLOW RATE: 0.6ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
COLUMN OVEN PROGRAM
Time Temp ºC
0 42
4 42
9 70
32 70
33 42
0 5 1 0 15 20 2 5 3 0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
20
23
24
25
HPLC PROGRAM
Time % Na315 % Na425 % Na640 % RG011
0 100 0 0 0
4.0 100 0 0 0
15.0 0 100 0 0
16.0 0 0 100 0
31.0 0 0 100 0
31.1 0 0 0 100
33.0 0 0 0 100
33.1 100 0 0 0
40 100 0 0 0