Pickering Laboratories Pinnacle PCX - Page 104

6.2-14
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
PEAK IDENTIFICATION
CONDITIONS FOR OXIDIZED SAMPLES 1154150T
Time (Min) Na270 % Na740 % RG011 %
0 100 0 0
14 100 0 0
32 20 80 0
32.1 0 100 0
56 0 100 0
56.1 0 0 100
58 0 0 100
58.1 100 0 0
70 100 0 0
3 Aspartic Acid
4 Threonine
5 Serine
6 Glutamic Acid
7 Proline
8 Glycine
9 Alanine
11 Valine
13 Isoleucine
14 Leucine
16 Phenylalanine
18 Lysine
19 Ammonia
20 Histidine
22 Arginine
23 Cysteic Acid
24 Methionine Sulfone
3
4
5
6
7
8
9
11
13
14
16
18
19
20
22
23
24
min
0 10 20 30 40 50
METHOD 9: 1154150T ISOTHERMIC OXIDIZED HYDROLYSATE SODIUM (4.0 x 150mm)
GUARD COLUMN: GARD COLUMN TEMP: 50°C HPLC FLOW RATE: 0.4ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul