6.2-15
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
3
4
5
6
7
8
9
10
11
12
13
14
15
16
18
19
20
21
22
min
0 10 20 30 40 50
*Protein
Hydrolysate
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
22
min
0 10 20 30 40 50
*Collagen
Hydrolysate
1 Methionine-D,L,-Sulfoxide
2 trans-4-Hydroxy-L-Proline
3 Aspartic Acid
4 Threonine
5 Serine
6 Glutamic Acid
7 Proline
8 Glycine
9 Alanine
10 Cystine
11 Valine
12 Methionine
13 Isoleucine
14 Leucine
15 Tyrosine
16 Phenylalanine
17 D,L & allo-Hydroxylysine
18 Lysine
19 Ammonia
20 Histidine
21 Tryptophan
22 Arginine
CONDITIONS FOR PROTEIN &
COLLAGEN HYDROLYSATE SAMPLES
Time (Min) Na315 % Na740 % RG011 %
0 100 0 0
10 100 0 0
30 0 100 0
53 0 100 0
53.1 0 0 100
55 0 0 100
55.1 100 0 0
67 100 0 0
METHOD 10: 1154150T ISOTHERMIC PROTEIN AND COLLAGEN HYDROLYSATE SODIUM (4.0 x 150mm)
GUARD COLUMN: GARD COLUMN TEMP: 48°C HPLC FLOW RATE: 0.4ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
PEAK IDENTIFICATION
To Next Page