6.2-16
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
METHOD 11: 1154150 ISOTHERMIC PROTEIN AND COLLAGEN HYDROLYSATE SODIUM (4.0 x 150mm)
GUARD COLUMN: GARD COLUMN TEMP: 48°C HPLC FLOW RATE: 0.40ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
Time(min) %1700-0112* %Na740 %RG011 Comment
0 100 0 0 inject
12 100 0 0 isocratic
34 0 100 0 linear gradient
53 0 100 0 isocratic
53.1 0 0 100 step gradient
55 0 0 100 isocratic
55.1 100 0 0 isocratic
67 100 0 0 re-equilibration
1
2
3
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
4
10 20 30 40 500
Collagen Hydrolysate
10 20 30 40 50
MINUTES
3
22
21
20
19
18
16
15
14
13
12
11
10
9
8
6
5
4
7
600
Protein Hydrolysate
* For use with columns with serial numbers after 1314
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