Pickering Laboratories Pinnacle PCX - Page 107

6.2-17
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
METHOD 12: 1193250 ISOTHERMIC PROTEIN HYDROLYSATE SODIUM (3.0 x 250mm)
GUARD COLUMN: GARD COLUMN TEMP: 48°C HPLC FLOW RATE: 0.30ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
Time(min) %Na328 %Na740 %RG011 Comment
0 100 0 0 inject
10 100 0 0 isocratic
32 0 100 0 linear gradient
56 0 100 0 isocratic
56.1 0 0 100 step gradient
58 0 0 100 isocratic
58.1 100 0 0 step change
70 100 0 0 re-equilibration
PEAK IDENTIFICATION
1. Methionine sulfoxide
2. Hydroxyproline
3. Aspartic acid
4. Threonine
5. Serine
6. Glutamic acid
7. Proline
8. Glycine
9. Alanine
10. Cystine
11. Valine
12. Methionine
13. Isoleucine
14. Leucine
15. Tyrosine
16. Phenylalanine
17. Hydroxylysines
18. Lysine
19. Ammonia
20. Histidine
21. Tryptophan
22. Arginine
23. Cysteic acid
24. Methionine Sulfone
25. Norleucine
10 20 30 40 50 600
3
7
16
15
14
13
12
11
10
9
8
6
5
4
20
19
18
22
21
MINUTES