6.3-3
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
FOR WATER SAMPLES
Sampling Protocol
To preserve the Carbamates in water, this procedure should be carried out in the field.
SAMPLING PROTOCOL for EPA Method 531.1 and 531.2
1. Add 1.8 ml of ChlorAC Buffer to each pre-cleaned 60 ml sample vial (see note about well and river waters!)
2. If the water sample is chlorinated, dechlorinate with 5 mg of Sodium thiosulfate per 60 ml sample.
3. Fill the sample vials with the dechlorinated water, seal, and mix well.
4. Refer to method EPA 531 or 531.2 for sample transportation and storage details.
Sample Preparation
Filter 2ml of sample through a 0.45m filter.
Inject 200-400l.
FOR STANDARDS AND BLANKS
Use 10 ml ChlorAC Buffer diluted to 1000 ml with HPLC-grade water.
Note: Well and river waters contain colloidal iron which would dissolve if samples are preserved prior
to filtration only to precipitate out again as the hydroxide in the reactor. For well and river waters, it is
recommended to filter the water first through a 0.45m filter, and then preserve with ChlorAC
™
.
Reagent Preparation
The two derivatization reagents required for carbamate analysis are a hydrolysis reagent (NaOH) and
o-phthalaldehyde reagent.
Note: During initial installation, the reagent bottles, lines, and pump should first be cleaned and primed with
methanol to reduce possible fluorescence background.
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