6.3-4
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
REAGENT 1, HYDROLYSIS REAGENT
Turn off the inert gas.
Thoroughly wash the two reagent reservoirs and then rinse with methanol. Wipe down the dip tubes with
methanol and a clean cellulose tissue.
The hydrolysis reagent does not require preparation. Pour the hydrolysis reagent (Cat. No. CB130 or CB130.2)
directly into the reagent reservoir for Reagent 1. It should be labeled Hydrolysis Reagent. Put the cap on the
reservoir. Close the vent valve.
The Hydrolysis reagent remains stable indefinitely.
Note: The preparation of the Hydrolysis Reagent by the user is not recommended because it is hard to obtain
NaOH of adequate purity.
REAGENT 2, OPA REAGENT
1. Pour 945ml of the OPA Diluent (Cat. No. CB910) into the reagent reservoir. Save approximately 5ml
for step 5.
2. Put the cap on the bottle, open the vent valve, and turn on the gas supply. Thoroughly de-aerate the contents
by sparging with inert gas. Continue bubbling for at least 10 minutes
3. Dissolve 100 mg of OPA (Cat. No. O120) in approximately 10 ml of HPLC-grade methanol in a clean, dry
container.
4. Turn off the gas supply and remove the cap from the bottle. Add the OPA solution to the deoxygenated
Diluent in the reservoir.
5. Dissolve 2 g of Thiofluor
™
(Cat. No. 3700-2000) in the reserved 5 ml of the OPA diluent from Step 1 and add
into the reservoir.
6. Replace the cap and turn on the gas flow. Continue sparging for another minute. Close the vent valve. Gently
swirl the reagent to complete the mixing.
Note: The preparation of the OPA Diluent by the user is not recommended because sodium borate (any
grades) contains excessive amounts of heavy metal contaminants and insoluble matter. These impurities will
eventually precipitate in the reactor and flowcell. The one year warranty does not cover damage caused by these
contaminants.
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