6.3-5
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
The OPA reagent is sensitive to air oxidation and degrades over time. When the OPA reagent reservoir is
maintained under inert gas pressure, the OPA reagent maintains its activity for one week without significant
loss of activity.
Post-column Conditions
These are the recommended post-column conditions for carbamate analysis. For the HPLC conditions, refer to
the section titled Sample Chromatograms and Gradient programs.
Reagent 1: CB130 or CB130.2, Hydrolysis Reagent (NaOH)
Reagent 2: o-Phthalaldehyde and Thiofluor
™
in CB910 Diluent
Pump 1 Flow Rate: 0.30 ml/min
Pump 2 Flow Rate: 0.30 ml/min
Reactor 1 Volume: 500 µl
Reactor 2 Volume: 100 µl
Reactor 1 Temp: 100°C
Reactor 2 Temp: Ambient
Analytical Procedure
Allow the column to equilibrate for about 20 minutes under initial conditions.
Inject 10µl of Carbamate Text Mixture (or the appropriate volume of your standard), and collect the first
chromatogram.
Sample Chromatograms and Gradient Programs
The Peak Names apply to all chromatograms in this section.
1. Aldicarb sulfoxide (Standak)
2. Aldicarb sulfone
3. Oxamyl (Vydate)
4. Methomyl (Lannate)
5. 3-Hydroxy carbofuran
6. Aldicarb (Temik)
7. Propoxur (Baygon)
8. Carbofuran (Furadan)
9. Carbaryl (Sevin)
10. 1-Naphthol
11. Methiocarb (Mesurol)
12. BDMC internal standard
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