6.4-4
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
Analytical and Post-column Conditions
These are the recommended conditions for glyphosate analysis using the 1954150 column and 1953020 guard
column.
Column Temperature: 55°C
HPLC Flow Rate: 0.4 ml/min
HPLC Program:
Times (min) %K200 %RG019 Comment
0 100 0 Inject
15.0 100 0 Isocratic
15.1 0 100 Regeneration
17 0 100 Isocratic
17.1 100 0 Step Change
27.0 100 0 Re-equilibration
The exact time of equilibration depends on the internal volume of your HPLC. When the baseline and column
pressure are stable for two minutes, the column has been re-equilibrated.
Post-Column Conditions:
Reagent 1: 100 µl of 5% NaOCl in GA116 Diluent
Reagent 2: o-Phthalaldehyde and Thiofluor
™
in GA104 Diluent
Pump 1 Flow Rate: 0.30 ml/min
Pump 2 Flow Rate: 0.30 ml/min
Reactor 1 Volume: 500 µl
Reactor 2 Volume: 100 µl
Reactor 1 Temp: 36°C
Reactor 2 Temp: Ambient
Analytical Procedure
Allow the column to equilibrate for about 20 minutes under initial conditions.
Inject 10µl of Glyphosate Text Mixture (or the appropriate volume of your standard), and collect the first
chromatogram.
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