6.4-3
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
Cap the reservoir, close the vent valve, and swirl the solution to mix it thoroughly.
Note: The hypochlorite concentration slowly decreases with time. This will manifest itself as a change in the
relative peak areas of glyphosate and AMPA. It will remain usable for several days, but we recommend you
calibrate daily.
Caution! Do NOT use calcium hypochlorite in the oxidizing reagent. This will cause plugging of the post-
column reactor. The one year warranty does not cover damage caused by calcium hypochlorite-based reagents.
The EPA Draft Method 547 is wrong on this point; Ca
3
(PO
4
)
2
is insoluble in water.
OPA REAGENT
1. Pour 945 ml of the OPA Diluent (Cat. No. GA104) into the reagent reservoir. Save approximately 5 ml for
step 5.
2. Put the cap on the bottle, open the vent valve, and turn on the gas supply. Thoroughly de-aerate the contents
by sparging with inert gas. Continue bubbling for at least 10 minutes.
3. Dissolve 100 mg of OPA (Cat. No. O120) in approximately 10 ml of HPLC-grade methanol in a clean, dry
container.
4. Turn off the gas supply and remove the cap from the bottle. Add the OPA solution to the deoxygenated
Diluent in the reservoir.
5. Dissolve 2 g of Thiofluor
™
(Cat. No. 3700-2000) in the reserved 5 ml of the OPA Diluent and add into the
reservoir.
6. Replace the cap and turn on the gas flow. Continue sparging for another minute. Close the vent valve. Gently
swirl the reagent to complete the mixing.
Caution! The preparation of the OPA Diluent by the user is not recommended because sodium borate (any
grades) contains excessive amounts of heavy metal contaminants and insoluble matter. These impurities will
eventually precipitate in the reactor and flowcell. The one-year warranty does not cover damage caused by
these contaminants.
Note: The OPA reagent is sensitive to air oxidation and degrades over time. When the OPA reagent reservoir
is maintained under inert gas pressure, the OPA reagent maintains its activity for up to one week without
significant loss of activity.
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