2. General
TECAN
2-18 Instructions for Use for sunrise Absorbance Reader No. 30008746 Rev. No. 1.4 2006-03
2.6 Instrument Features
Microplates can be measured using the following features:
• Various measurement modes
• Single or dual wavelength measurements
• Microplate shaking
2.6.1 Measurement Modes
The instrument can be set to use the following measurement modes:
Normal The plate transport is moved quickly under the measurement
diodes so that a fast measurement is obtained. Each well is
measured at three points, 8 times for each point.
Accuracy The plate transport is moved very slowly under the measurement
diodes so that a very accurate measurement is obtained. Each well
is measured at three points, 55 times for each point.
Center This option measures the optical density only in the center of each
well. It is recommended for U-bottom wells or for liquids with high
meniscus. Each well is measured at one point, 22 times.
With the Normal and Accuracy measurement modes, the optical density is
measured at three positions across the wells and the average measured optical
density value from the three measurements is used as the optical density of the
well.
Note
The accurate measurement cycle should always be used when measuring
high optical densities.
The Center measurement mode should be used if the liquid in the microplate
produces a high meniscus, as an incorrect optical density could be obtained if the
optical density is measured at three positions. If an agglutination measurement is
performed, up to 40 measurement positions per well are used.
Note
For more information about setting the measurement mode,
see 3.8 Defining the Instrument Settings.
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