TECAN
7. Quality Control
2006-03 Instructions for Use for sunrise Absorbance Reader No. 30008746 Rev. No. 1.4 7-3
Microplates
1. The instrument can be used with those types of microplates which are
described in the specifications in chapter 2.3.8 Microplates. The best results
are obtained when clear flat-bottom microplates are used.
Depending on the type of microplate being used, the measurement result
may vary.
Take care especially when using round bottom plates or the strip
frames because it is possible that the measurement results might differ
slightly to what is described in the specifications.
Make sure that the type of microplate used with the
sunrise Absorbance
Reader is suitable for the respective application.
2. Use only perfectly clean microplates.
3. Do not allow dust to settle onto the solutions or the microplate, if the
microplate is left to stand for a time before the measurement.
It is recommended to use a cover for protection.
4. Inaccuracies in the amount of solution pipetted has a greater effect on the
results obtained, when small amounts of solutions are used.
It is recommended that a minimum of 200 ml is used in each well.
5. The form of the meniscus of the solution can cause inaccuracies in the
results, particularly if small amounts of solution are used (see 7.3.3 High
Meniscus Liquids).
Caution
Make sure the suitability of the microplate used in combination with the
amount of solution, meniscus properties and the measurement mode is
appropriate for the current application.
7.2.3 Self Check Procedure
Before each microplate is measured, the Self Check calibration procedure is
performed to ensure that the instrument is working correctly and to calibrate the
optical system.
When the self check procedure starts, a digital value for each measurement
channel is taken without the lamp and with the lamp on, using each of the
selected measurement filters.
A calibration curve for each measurement channel is calculated.
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