7. Quality Control
TECAN
7-8 Instructions for Use for sunrise Absorbance Reader No. 30008746 Rev. No. 1.4 2006-03
7.3.4 Instrument Precision with Liquid Samples
This procedure can be used to check the precision of the measurements from
one microplate to another.
Fill a new microplate with a freshly prepared Methyl Orange in 0.1 % Tween 20
solution, use different dilutions of the solution in each well so that a range of
optical densities is obtained. Make sure that the wells contain at least 200 ml.
Define a test run using the 492 nm filter and then measure the microplate at least
three times.
For each well calculate the following:
• the average OD value
• the highest and lowest values
• the difference between the average, highest and lowest values
Example for a standard instrument
Readings 0.000 to 2.000 OD
The difference between the average and the highest and lowest values for the
same well should be within +/- (1.0 % + 0.010 OD)
e.g. 1.000 +/- 0.020 OD
Readings 2.001 to 3.000 OD
The difference between the average and the highest and lowest values for the
same well should be within +/- (1.5 % + 0.010 OD)
e.g. 2.400 +/- 0.046 OD
Readings above 3.000 OD
Readings above 3.000 OD are only used as an indication and the precision
cannot be guaranteed.
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