tecan sunrise - Page 79

TECAN
7. Quality Control
2006-03 Instructions for Use for sunrise Absorbance Reader No. 30008746 Rev. No. 1.4 7-7
Agglutination Method
For agglutination measurements, an external software package must be used.
Depending on the software used, the
sunrise Absorbance Reader must be set to
sunrise or SPECTRA mode using the sunrise Instrument Settings software. The
sunrise Absorbance Reader is able to read up to 40 measurement points per
well.
Note
Instruments with option tuneable wavelength selection must not be used
for agglutination measurements.
After the microplate has been measured, select a well and zoom it so that it is
printed in large scale.
Normal Liquid Liquid with High Meniscus
1
10 20 30 40
0%
50%
100%
0%
50%
100%
RRCC
Measurement
1
10 20 30 40
0%
50%
100%
0%
50%
100%
RRCC
Measurement
If the printout has less than sixteen measurement points in the middle of the well,
that are at the same level, use the center measurement.
Manual Method
If the instrument is not able to perform agglutination measurements, measure the
microplate five times.
Then rotate the microplate by 180 ° and then measure the microplate five times.
For a number of wells, calculate the average optical density value from all the
measurements for these wells.
Compare the average value against the highest and lowest measured values.
Example
Measured Values
0.945, 0.956, 0.937, 0.926, 0.971, 0.936, 0.961, 0.939, 0.942, 0.938 OD
Average = 0.945 OD, highest value = 0.971 OD, lowest value = 0.926 OD
Tolerances for standard instruments: 0.945 +/- (0.5 % + 0.005 OD) =
0.945 +/- 0.010 OD
Highest value within tolerance = 0.955 OD
Lowest value within tolerance = 0.935 OD
Make sure that the values are within the allowed tolerances. If not, use the center
measurement mode.
Repeat the procedure using the center measurement, to ensure that the
measured values are now within the required tolerances.