3
System Shutdown, Startup, and Reset
Starting Up the System after a Complete Shutdown
56 TSQ Series Hardware Manual Thermo Scientific
Setting Up Conditions for Operation
To set up your TSQ mass spectrometer for operation
1. Before you begin data acquisition with your TSQ system, allow the system to pump down
for at least one hour. Operation of the system with excessive air and water in the vacuum
manifold can cause reduced sensitivity, tuning problems, and reduced lifetime of the
electron multiplier.
2. Ensure that the argon pressure and nitrogen pressure are within the operational limits
[argon: 135 ±70 kPa (20 ±10 psig), nitrogen: 690 ±140 kPa (100 ±20 psig)].
3. In the EZ Tune window, choose View > Instrument Status and look at the Instrument
Status view. Check that the ion gauge pressure is below about 5 × 10
–
6
Torr, and that the
forepump pressure is below about 50 mTorr (with the ion transfer tube sealed with a
septum cap).
4. Remove the septum cap from the orifice of the ion transfer tube.
5. Set up the API source for operation as described in the Ion Max and Ion Max-S API Source
Hardware Manual, HESI-II Probe User Guide, H-ESI Probe User Guide, Nanospray Ion
Source Operator’s Manual, and TSQ Series Getting Started Guide.
Note Air in the argon line must be purged or given sufficient time to be purged for
normal TSQ mass spectrometer performance.
Note You do not need to calibrate and tune the TSQ mass spectrometer each time
you restart the TSQ system.
Calibration parameters are instrument parameters that affect the mass accuracy and
resolution. Tune parameters are instrument parameters that affect the intensity of the
ion signal. You must tune and calibrate the TSQ mass spectrometer (that is, optimize
the tune and calibration parameters) perhaps once a quarter. Refer to “Automatic
Tuning and Calibrating in the ESI/MS Mode” in the TSQ Series Getting Started Guide
for a procedure for tuning and calibrating the TSQ mass spectrometer.
You must optimize the tune parameters (or change the Tune Method) whenever you
change the type of experiment. For procedures on how to optimize the tune
parameters for an ESI or APCI experiment, refer to “Optimizing the Mass
Spectrometer with Your Compound in ESI/MS/MS Mode” or “Optimizing the
Mass Spectrometer with Your Compound in APCI/MS/MS Mode” in the TSQ Series
Getting Started Guide.
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