4
Daily Operation
Things to Do After Operating the TSQ System
Thermo Scientific TSQ Series Hardware Manual 67
5. Set up the ESI source as follows:
a. In the EZ Tune window, click on the Ion Source button to display the Ion Source
Devices dialog box.
b. Set the sheath gas flow rate to 30:
Select Sheath Gas Pressure in the device list, and then type 30 in the box and click
Apply.
c. Set the auxiliary gas flow rate to 5:
Select Aux Valve Flow in the device list, and then type 5 in the box and click Apply.
d. Set the ESI spray voltage to 0:
Select Spray Voltage in the device list, and then type 0 in the box and click Apply.
6. Set up and start a flow of 50:50 methanol/water solution from the LC to the API source
as follows:
a. In the EZ Tune window, choose Setup > Inlet Direct Control. The Inlet Direct
Control view appears.
b. Click the LC’s tab.
c. Set the flow rate to a value that is typical for your experiments.
d. Set the solvent proportions to 50% methanol and water.
e. Click (Start) to start the LC pump.
7. Let the solution flow through the sample transfer line, sample tube, and API probe for
15 minutes. After 15 minutes, turn off the flow of liquid from the LC to the API source,
as follows: Leave the API source (including the APCI vaporizer, sheath gas, and auxiliary
gas) on for an additional 5 minutes. Click (Stop) to stop the LC pump.
8. After 5 minutes, turn off the API source by placing the mass spectrometer in standby:
From the EZ Tune window, choose Control > Standby (or click the On/Standby
button) to put the mass spectrometer in standby.
Go to the next topic, “Placing the System in Standby Condition.”
Placing the System in Standby Condition
To place the TSQ system in standby condition
1. From the EZ Tune window, choose Control > Standby (or click the On/Standby
button) to put the mass spectrometer in standby. The System LED on the front panel of
the mass spectrometer is illuminated yellow when the system is in standby.
2. Leave the mass spectrometer power on.
3. Leave the LC power on.
4. Leave the autosampler power on.
5. Leave the data system power on.
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