2
Scan Parameters
Scan Rates for the Ion Trap Detector
12 Orbitrap Fusion Series Hardware Manual Thermo Scientific
Single-stage full-scan analysis is a useful tool for qualitative and quantitative analysis. Use
single-stage full-scan experiments to determine the molecular weight and intensity of
compounds present in the mass spectrum.
MS
2
Scan and MS
n
Scan
An MS
2
Scan selects the MS/MS mass analysis. An MS
n
Scan typically involves 2 to 10 stages
of mass analysis (scan power of n =2 to n = 10). Each stage of mass analysis where n >1
includes an ion selection step. As you raise the scan power, you can obtain more structural
information that can be useful in structure elucidation of compounds of interest. The
Orbitrap Fusion Series MS has several advanced features that make its MS
n
capabilities
extremely powerful.
In an MS/MS scan, precursor ions fragment into product ions. The MS/MS scan type
experiment can use a full-scan or a defined scan range.
• First stage of mass analysis—You can set either the Quadrupole or the Ion Trap to select
the ions for MS/MS analysis. The mass spectrometer then transfers the selected ions to
the IRM for HCD or to the linear ion trap (LIT) for CID or ETD.
• Second stage of mass analysis—The mass spectrometer transfers the product ions to either
the Orbitrap or the LIT for detection.
In the n
th
stage of mass analysis, subsequent stages of product ion selection occur in the LIT.
SIM Scan
The selected ion monitoring (SIM) scan type is a single-stage (scan power of n =1) technique
that monitors a particular ion or set of ions. In a SIM scan, the mass spectrometer selects ions
in the defined mass-to-charge ratio range, and ejects all other ions by using either the
quadrupole or the LIT analyzer. The Orbitrap or the LIT then detects the selected ions to
produce a SIM mass spectrum.
Scan Rates for the Ion Trap Detector
With the Orbitrap Fusion Series MS, you can use five scan rates: Turbo, Rapid, Normal,
Enhanced, and Zoom. For information about these scan rates, refer to the Tune Help.
Tab le 5 lists the scan rates and their peak widths (resolutions) for the Orbitrap Fusion Series
MS. For additional information about the scan rates, refer to the data system Help.
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