6
System Shutdown, Startup, and Reset
Resetting the Mass Spectrometer
Thermo Scientific Orbitrap Fusion Series Hardware Manual 55
• After 3 minutes, the System LED turns yellow to indicate the software transfer from
the data system to the mass spectrometer is complete and that the mass spectrometer
is in standby mode. When you change the mode from standby to on, the System
LED turns green to indicate that the mass spectrometer is functional and the high
voltages are on.
Starting the Autosampler
Turn on the autosampler by using its on/off power switch. If necessary, configure the
autosampler. For procedures for placing sample vials, preparing solvent and waste containers,
installing syringes, and so on, refer to the autosampler manual. The Orbitrap Fusion Series
Getting Connected Guide provides procedures for connecting the mass spectrometer to the
autosampler by using a contact closure cable.
Resetting the Mass Spectrometer
In the unlikely event that communication is lost between the mass spectrometer and data
system computer, you can reset the mass spectrometer by using the reset button located on the
left-side communications panel.
The following procedure assumes that power to the mass spectrometer and data system
computer are on and that both are operational. If the mass spectrometer, data system
computer, or both are off, see “Starting the System after a Complete Shutdown.”
IMPORTANT On the front panel, the Vacuum LED illuminates green only when
these gauges are on and register below the indicated pressure threshold:
• Source Pressure gauge—Below 3.0 Torr (Orbitrap Fusion MS) or 4.5 Torr
(Orbitrap Fusion Lumos MS) as applicable
• Ion Gauge Pressure gauge—Below 1.5 × 10
–4
Torr
• UHV Pressure gauge (Orbitrap)—Below 5 × 10
–8
Torr
Although you can calibrate the mass spectrometer after the vacuum LED turns
green, you must allow the mass spectrometer’s vacuum system to stabilize
completely, which takes approximately 15–24 hours of continuous pumping, to
ensure that the calibrations are correct.