Waters 2414 - Solvent degassing

June 11, 2015, 715004754 Rev. A
Page 59
4.3 Solvent degassing
Using degassed solvents is the most important step in solvent preparation. Degassing
provides:
• Stable baselines and enhanced sensitivity
• Reproducible retention times
• Stable pump or solvent delivery system operation
Table 4–1: Refractive indices of common solvents
Solvent RI Solvent RI
Fluoroalkanes 1.25 Tetrahydrofuran (THF) 1.408
Hexafluoroisopropanol
(HFIP)
1.2752 Amyl alcohol 1.410
Methanol 1.329 Diisobutylene 1.411
Water 1.33 n-Decane 1.412
Acetonitrile 1.344 Amyl chloride 1.413
Ethyl ether 1.353 Dioxane 1.422
n-Pentane 1.358 Ethyl bromide 1.424
Acetone 1.359 Methylene chloride 1.424
Ethanol 1.361 Cyclohexane 1.427
Methyl acetate 1.362 Ethylene glycol 1.427
Isopropyl ether 1.368 N,N-dimethyl formamide
(DMF)
1.428
Ethyl acetate 1.370 N,N-dimethyl acetamide
(DMAC)
1.438
1-Pentene 1.371 Ethyl sulfide 1.442
Acetic acid 1.372 Chloroform 1.443
Isopropyl chloride 1.378 Ethylene dichloride 1.445
Isopropanol 1.38 Carbon tetrachloride 1.466
n-Propanol 1.38 Dimethyl sulfoxide (DMSO) 1.477
Methylethylketone 1.381 Toluene 1.496
Diethyl amine 1.387 Xylene ~1.50
n-Propyl chloride 1.389 Benzene 1.501
Methylisobutylketone 1.394 Pyridine 1.510
Nitromethane 1.394 Chlorobenzene 1.525
1-Nitropropane 1.400 o-Chlorophenol 1.547
Isooctane 1.404 Aniline 1.586
Cyclopentane 1.406 Carbon disulfide 1.626