Waters 2690 - Equilibrating Solvents the Vacuum Degasser; Priming the Plunger Seal Wash Pump; To Prime the Plunger Seal Wash Pump

Preparing the Separations Module for Operation 23
4. Gently shake the filters in the reservoirs to remove any bubbles that may have
formed.
5. Enter the flow rate and the duration of the prime (using the composition entered
in the Status screen), then press
OK
. The solvent management system begins
to operate.
At the end of the time period you specify, the solvent management system
returns to previous conditions and the Separations Module enters the Idle mode.
6. Repeat steps 1–5 for each solvent, as appropriate.
Equilibrating Solvents the Vacuum Degasser
Solvents are equilibrated in their respective degasser chamber by setting a zero flow rate
for a short time period. The steps that follow describe this process.
To equilibrate solvents the in-line vacuum degasser after you have wet primed the system:
1. Enter the initial solvent composition for the run in the Status screen.
2. Set the Degasser Mode parameter to
Normal
or
Continuous
.
3. Press the
Direct Function
screen key. The Direct Functions menu appears
(Figure 3-4
).
4. Select
Wet Prime
, then press
Enter
. The Wet Prime dialog box appears
(Figure 2-11
).
5. Enter a flow rate of
0.000
mL/min and a time of
5
min. At the end of the time
period, the solvents are degassed and the Separations Module enters the Idle
mode.
The solvents in the in-line vacuum degasser are now equilibrated. Prime the
plunger seal wash pump as described below.
Priming the Plunger Seal Wash Pump
For reversed-phase HPLC applications, use an aqueous plunger seal-wash solution with
enough organic content to inhibit bacterial growth. For example, use an 80%water:20%
methanol solution or an 80% water:20% acetonitrile solution, depending on your
application. For GPC separations, use a 50% methanol:50% water seal wash solution. If
required, use an intermediate solution to prevent immiscibility or precipitate problems
when you switch between GPC and reversed-phase analytical solvents.
To prime the plunger seal wash pump:
1. Press the
Diag
screen key in the Main screen. The Diagnostics screen appears
(Figure 2-12
). Refer to the Waters 2690 Separations Module Operator’s Guide ,
Section 8.3, Performing Main Diagnostics, for more information on the Diagnostics
screen.