Waters 2690 - Before Powering off; Powering off; To Power off the Separations Module

48 Powering Off the Separations Module
5
Powering Off the Separations
Module
Before Powering Off
Before you power off the Separations Module, remove any buffered mobile phase present
in the fluidic path.
To remove buffered mobile phase from the fluidic path of the Separations Module:
1. Replace the buffered mobile phase with HPLC-quality water and prime the system
for 10 minutes at 3 mL/min (see the Section 2.3.2,
Priming the Solvent Management
System, the “Wet Prime” discussion).
2. Perform three injector purge cycles to ensure that the sample loop is clean (see
Section 2.4.1,
Purging the Sample Management System).
3. Replace the plunger seal wash solvent with a fresh solution of 80% water:20%
methanol or a miscible solvent, and prime the plunger seal wash pump (see
Section 2.3.2,
Priming the Solvent Management System, the “Priming the Plunger
Seal Wash Pump” discussion).
4. Replace the water mobile phase with a solution of 90% methanol:10% water,
and flush the system for 10 minutes at 3 mL/min.
5. Replace the needle-wash solvent with a solution of 90% methanol:10% water or
a miscible solvent, and perform a needle-wash prime (see Section 2.4.2,
Priming
the Needle-Wash Pump).
Powering Off
To power off the Separations Module:
1. Ensure that the system has been flushed properly (see the “
Before Powering Off”
discussion above).
2. Press the power switch to the
0
(Off) position. The time and date of power-off are
entered in the log file.
STOP
Attention:
To avoid damaging your column, remove the column before you perform the
following procedure.