November 16, 2016, 715004521 Rev. C
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2.2 Starting the console software .................................................................................................... 23
2.2.1 Starting the console ....................................................................................................... 24
2.3 Priming and equilibrating the system ........................................................................................ 25
2.3.1 Priming the system ........................................................................................................ 25
2.3.2 Equilibrating the system................................................................................................. 26
2.4 Observing the sample-injection sequence ................................................................................ 26
2.5 Performing column screening .................................................................................................... 32
2.6 Shutting down the ACQUITY UPC
2
system .............................................................................. 33
3 Method parameters ............................................................................................... 35
3.1 Creating methods in Empower software .................................................................................... 35
3.2 Creating instrument methods for the UPC
2
modules ................................................................ 35
3.2.1 ccBSM instrument method settings ............................................................................... 35
3.2.2 SM-FL instrument method settings................................................................................ 36
3.2.3 Instrument method settings for the column manager .................................................... 36
3.2.4 Instrument method settings for the ccManager ............................................................. 36
3.2.5 ACQUITY UPC
2
PDA instrument method settings ........................................................ 38
3.3 Creating methods in MassLynx software ................................................................................... 38
4 External connections ............................................................................................ 39
4.1 External connections ................................................................................................................. 40
4.2 Connecting Ethernet cables ...................................................................................................... 40
4.2.1 Connecting external signal cables ................................................................................. 41
4.2.2 Connecting the UPC
2
PDA detector signal cables ........................................................ 44
4.3 Connecting the system to an electricity source ......................................................................... 44
5 Splitter configurations .......................................................................................... 47
5.1 Splitter components ................................................................................................................... 47
5.1.1 Components of the core 1, 2, and 3 splitters ................................................................. 47
5.1.2 Components of the core 4 splitter.................................................................................. 49
5.1.3 Isocratic solvent manager splitter .................................................................................. 50