Waters ACQUITY UPC2 - Method Parameters; Creating Methods in Empower Software; Creating Instrument Methods for the UPC Modules; Ccbsm Instrument Method Settings

November 16, 2016, 715004521 Rev. C
Page 35
3 Method parameters
Creating methods for UPC
2
normal-phase chromatography is similar to creating them for
HPLC normal-phase chromatography, the sole difference being the replacement of water
with liquid CO
2
.
3.1 Creating methods in Empower software
In Empower software, you first create an instrument method in a method set to specify your
instrument parameters. Create a processing method to specify how you want to process and view
the results.
See also: The “Getting Started” section in the Empower software online Help, the topic “Using a
quick-start guided tour to learn about Empower”. Click on the Empower Quick Start Tour link. Also
see the topics in the “Define Methods” section in the Empower software online Help.
3.2 Creating instrument methods for the UPC
2
modules
Configure an instrument method for each of the following ACQUITY UPC
2
system modules that
are part of your system:
• ccBSM
•SM-FL
• CM-A or CM-30S
• ccManager
•PDA
• Mass spectrometers
3.2.1 ccBSM instrument method settings
Follow these guidelines when setting up a method for the ccBSM:
• Flow-rate range is 0.000 to 4.000 mL/min.
• At a flow rate of 3.25 mL/min or less, maximum pressure 41,369 kPa (413 bar, 6000 psi).
• Solvent A is always liquid CO
2.
• Solvent B can be one of four co-solvents.