Waters ACQUITY UPC2 - ACQUITY UPC System; Using ACQUITY UPC for Normal-Phase Chromatography

November 16, 2016, 715004521 Rev. C
Page 13
1 ACQUITY UPC
2
system
The ACQUITY
®
UPC
2®
(Ultra Performance Convergence Chromatography) system uses
liquid carbon dioxide (CO
2
) as the primary mobile phase for normal-phase, chiral, and
achiral separations.
You control the system using the driver pack software that you access through one of these
Waters’ data systems: Empower
®
software or MassLynx
®
software.
1.1 Using ACQUITY UPC
2
for normal-phase
chromatography
ACQUITY UPC
2
chromatography is a normal-phase chromatographic technique that offers these
advantages over traditional HPLC normal-phase chromatography:
• Uses liquid CO
2
, a chemically pure, stable, and nonpolar solvent, as the primary mobile
phase.
• Combines liquid CO
2
with one or more organic solvents, typically alcohols such as methanol
and isopropanol, to increase the solvent strength for separating polar compounds.
• Minimizes health, safety, and environmental concerns associated with chemical solvents.
• Runs at higher flow rates (3 to 5 times higher than HPLC for the columns of the same
dimension) because of its high diffusivity.
• Produces lower pressure drops across the column and faster separations, because of the
mobile phases’s low viscosity.
Figure 1–1: Pressure-temperature phase diagram for CO
2
75 bar
Pressure
Temperature