November 16, 2016, 715004521 Rev. C
Page 27
Figure 2–4: Step 2: SM-FL aspirates the sample (PLNO)
The SM-FL moves the needle to the programmed vial position and aspirates an air gap. It then
aspirates pre-sample buffer from the vial, the programmed amount of sample from the vial,
post-sample buffer from the vial, and then returns the needle to above the injection port, aspirating
a final air gap while in that position.
Auxiliary valve (load position)
Waste
Waste
Metering syringe
Injection valve (inject position)
Sample
Liquid mobile phase (CO
2
)
Gas mobile phase (CO
2
)
Air gap
Buffer volume
Sample
Weak wash
From pump
To col umn
Sample loop
Sample
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