Pannoramic SCAN II 2.2.0 User’s Guide 5.1.1 Profile Settings Panel
• Source: Brightfield preview – a normal type of illumination in the microscope for scanning
the slide. This option is recommended when scanning in Brightfield mode.
• Thresholding: The manually specified value is used for thresholding during scanning. Preview
image of the loaded slide is displayed in the Preview window, and when modifying any of the
parameter values, it will have effect on the preview image. If the threshold value presented
on the Microscope Controller preview window is modified, this value is automatically applied
by the profile in the Profile Settings panel.
◦ Specimen threshold
This value is definable manually: This sets the sensitivity of the specimen detection. To
set the desired threshold value manually, change the setting in the Specimen threshold
level selection box in the range of 0-255. The default value is 220. Smaller values result in
a more sensitive scanning, meaning that more areas will be digitized, however, this may
also include specks or other imperfections of the slide. Greater values result in a less
sensitive scanning, but it is possible that some of the smaller specimen areas will not be
digitized.
◦ Auto Threshold
By clicking the specimen will automatically be detected in the preview image.
Caution!
Make sure that the detection result is acceptable for you. Otherwise,
set the threshold value manually or append missing areas with the
help of appropriate functions in the Preview window.
◦ Use marker with threshold
If the specimen cannot be identified as required for digitization, (for example, weak
contrast), it can help to encircle completely the specimen with a black marker pen. You
have to draw a full circle with the pen to enable the detection. Marker pen detection will
make specimen detection easier by limiting the scanned area. If this option is selected,
only the area encircled with a marker pen will be scanned. You can define the threshold
level by changing the threshold value in the Use marker with threshold selection box in
the range of 0-255. This sets the sensitivity of the specimen detection inside the marker
area. To set the desired threshold value manually, change the setting in the Specimen
threshold selection box in the range of 0-255. The default value is 105.
◦ Everything within marker
To scan everything within the marked area, check the Everything within marker selection
box.
Alternatively, click in the preview window to activate this function.
January 24, 2020 - Rev. 2 3DHISTECH Ltd. 56(156)
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