Rev. Date: 30-Jan-12 Art: 714185-00N Celite ACT - 7
The analyzer should remain on a level surface with the display facing up during testing. If the analyzer is not
level, the ACT result may be affected by more than 10%. A level surface includes running the handheld in
the downloader/recharger.
Hemodilution may affect test results.
Platelet dysfunction, hereditary or acquired, may affect the results of this test. This includes the
administration of pharmacological compounds known as platelet inhibitors which affect platelet function.
Factor deficiencies, dysprothrombinemias, other coagulopathies, and other pharmacological compounds
may also affect the results of this test.
The i-STAT ACT test is not affected by hematocrit in the range of 20 - 70%, fibrinogen concentration in the
range from 100 - 500 mg/dL, or sample temperature from 15 - 37ºC.
Specimen Collection and Preparation
The i-STAT
Celite
ACT test can be performed using venous or arterial samples.
Venipunctures and Arterial Punctures
• Collectiontechniqueresultingingoodbloodflowmustbeused.
• Thesamplefortestingshouldbedrawnintoaplastic collection device (either a plastic
syringe or plastic evacuated tube).
• Thecollectiondevicecannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
• Thecollectiondevicecannotcontainclotactivatorsorserumseparators.
• Thesampleshouldbeimmediatelydispensedintothesamplewellofacartridge.
• Ifasecondmeasurementisrequired,afreshsamplemustbeobtained.
Note: Some experts recommend drawing and discarding a sample of at least 1 mL prior to drawing
sample for coagulation testing.
5
Indwelling line
• Fluiddripthroughthelinemustbediscontinued.
• If blood must be drawn from an indwelling line, possible heparin contamination and specimen dilution
should be considered. The line should be flushed with 5 mL of saline and the first 5 mL of blood or
six dead space volumes should be discarded.
• Withdrawthesamplefortestingintoafreshplastic syringe.
• Thecollectionsyringecannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
• Thesampleshouldbeimmediatelydispensedintothesamplewellofacartridge.
• Ifasecondmeasurementisneeded,drawafreshsample.
Extracorporeal line
• Flushtheextracorporealbloodaccesslinebywithdrawing5mLofbloodintoasyringeanddiscardthe
syringe.
• Withdrawthesamplefortestingintoafreshplastic syringe.
• Thecollectionsyringecannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
• Thesampleshouldbeimmediatelydispensedintothesamplewellofacartridge.
• Ifasecondmeasurementisneeded,drawafreshsample.
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